ImageJ宏在ROI分析过程中出现循环错误的技术求助

我正在针对cFos分析处理共聚焦显微镜图像：从最大投影（maxstacked）图像中提取DAPI的ROI，再放大该ROI以检测转基因标记Piezo1的表达水平，以此验证Piezo1的表达是否与cFos水平升高相关。

此前我通过两次不同处理DAPI图像提取ROI的方法可正常运行，但存在两组ROI顺序可能不一致的问题。于是我尝试直接放大DAPI提取的ROI，却遇到了循环分析故障：宏陷入无限运行状态，重复保存同名文件，还会跳过文件数组中的部分文件。

存在问题的宏代码 #

dir1 = getDirectory("Choose Source Directory ");
dir2 = getDirectory("Choose Destination Directory ");
list = getFileList(dir1);
Array.sort(list);
run("Clear Results");
setBatchMode(true);
for (i=0; i<list.length; i++) {
    if (endsWith(list[i],"tif")) {
    showProgress(i+1, list.length);
    open(dir1+list[i]);
    title = getTitle();
    saveFilename = replace(list[i], "_Maxstacked.tif","");

    selectWindow(title);     
    run("Duplicate...", "title=DAPI duplicate channels=4");;
    
    selectWindow("DAPI");
    run("Subtract Background...", "rolling=80");     
    run("Gaussian Blur...", "sigma=1");
    run("8-bit");
    run("Auto Threshold", "method=Li white");
    run("Convert to Mask");
    run("Minimum...", "radius=8");
    run("Maximum...", "radius=9");
    run("Watershed");
    run("Analyze Particles...", "size=20-1000 display exclude clear include overlay add");
    roiN = roiManager("count");
    if (roiN > 0) {
        roiManager("Save", dir2+saveFilename+"_DAPI+_RoiSet.zip");
        for(i=0; i<roiN; i++) {
            roiManager("Select", i);
            run("Enlarge...", "enlarge=2.7");
            roiManager("Update");
            }
        roiManager("Save", dir2+saveFilename+"_DAPI+_for_Piezo1_RoiSet.zip");
        roiManager("Delete");
        roiManager("Delete");
        }    

    close("*");
    }
    }

可正常运行的宏代码 #

dir1 = getDirectory("Choose Source Directory ");
dir2 = getDirectory("Choose Destination Directory ");
list = getFileList(dir1);
Array.sort(list);
run("Clear Results");
setBatchMode(true);
for (i=0; i<list.length; i++) {
    if (endsWith(list[i],"tif")) {
    showProgress(i+1, list.length);
    open(dir1+list[i]);
    title = getTitle();
    saveFilename = replace(list[i], "_Maxstacked.tif","");

    selectWindow(title);     
    run("Duplicate...", "title=DAPI duplicate channels=4");
    selectWindow(title);    
    run("Duplicate...", "title=DAPI_for_Piezo1 duplicate channels=4");
    
    selectWindow("DAPI");
    run("Subtract Background...", "rolling=80");     
    run("Gaussian Blur...", "sigma=1");
    run("8-bit");
    run("Auto Threshold", "method=Li white");
    run("Convert to Mask");
    run("Minimum...", "radius=8");
    run("Maximum...", "radius=9");
    run("Watershed");
    run("Analyze Particles...", "size=20-1000 display exclude clear include overlay add");
    roiN = roiManager("count");
    if (roiN > 0) {
        roiManager("Save", dir2+saveFilename+"_DAPI+_RoiSet.zip");
        roiManager("Delete");
        }    
    
    selectWindow("DAPI");
    run("Subtract Background...", "rolling=80");     
    run("Gaussian Blur...", "sigma=1");
    run("8-bit");
    run("Auto Threshold", "method=Li white");
    run("Convert to Mask");
    run("Minimum...", "radius=8");
    run("Maximum...", "radius=16");
    run("Watershed");
    run("Analyze Particles...", "size=20-1000 display exclude clear include overlay add");
    roiN = roiManager("count");
    if (roiN > 0) {
        roiManager("Save", dir2+saveFilename+"_DAPI+_for_Piezo1_RoiSet.zip");
        roiManager("Delete");
        }    

    close("*");
    }
    }

内容的提问来源于stack exchange，提问作者postdoc0115