发布MVC项目至IIS8遇程序集加载权限拒绝错误求助

从几天前开始，发布到局域网内Server 2012R2 IIS8的任何MVC项目都会返回权限拒绝错误。已存在的站点运行正常，项目在本地开发电脑上构建和运行也没问题。即使发布未修改的项目到新站点，或覆盖现有站点，都会触发相同错误，且正常站点与异常站点的文件内容看起来完全一致。

错误日志 #

Server Error in '/mvcappname' Application.
Could not load file or assembly 'RSStatus' or one of its dependencies. Access is denied.
Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.

Exception Details: System.IO.FileLoadException: Could not load file or assembly 'mvcappname' or one of its dependencies. Access is denied.

Source Error:

An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below.

Assembly Load Trace: The following information can be helpful to determine why the assembly 'mvcappname' could not be loaded.


WRN: Assembly binding logging is turned OFF.
To enable assembly bind failure logging, set the registry value [HKLM\Software\Microsoft\Fusion!EnableLog] (DWORD) to 1.
Note: There is some performance penalty associated with assembly bind failure logging.
To turn this feature off, remove the registry value [HKLM\Software\Microsoft\Fusion!EnableLog].

Stack Trace:
[FileLoadException: Could not load file or assembly 'mvcappname' or one of its dependencies. Access is denied.]
   System.Reflection.RuntimeAssembly._nLoad(AssemblyName fileName, String codeBase, Evidence assemblySecurity, RuntimeAssembly locationHint, StackCrawlMark& stackMark, IntPtr pPrivHostBinder, Boolean throwOnFileNotFound, Boolean forIntrospection, Boolean suppressSecurityChecks) +0
   System.Reflection.RuntimeAssembly.InternalLoadAssemblyName(AssemblyName assemblyRef, Evidence assemblySecurity, RuntimeAssembly reqAssembly, StackCrawlMark& stackMark, IntPtr pPrivHostBinder, Boolean throwOnFileNotFound, Boolean forIntrospection, Boolean suppressSecurityChecks) +232
   System.Reflection.RuntimeAssembly.InternalLoad(String assemblyString, Evidence assemblySecurity, StackCrawlMark& stackMark, IntPtr pPrivHostBinder, Boolean forIntrospection) +113
   System.Reflection.RuntimeAssembly.InternalLoad(String assemblyString, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection) +23
   System.Reflection.Assembly.Load(String assemblyString) +35
   System.Web.Configuration.CompilationSection.LoadAssemblyHelper(String assemblyName, Boolean starDirective) +49

[ConfigurationErrorsException: Could not load file or assembly 'mvcappname' or one of its dependencies. Access is denied.]
   System.Web.Configuration.CompilationSection.LoadAssemblyHelper(String assemblyName, Boolean starDirective) +762
   System.Web.Configuration.CompilationSection.LoadAllAssembliesFromAppDomainBinDirectory() +259
   System.Web.Configuration.CompilationSection.LoadAssembly(AssemblyInfo ai) +167
   System.Web.Compilation.BuildManager.GetReferencedAssemblies(CompilationSection compConfig) +238
   System.Web.Compilation.BuildManager.GetPreStartInitMethodsFromReferencedAssemblies() +78
   System.Web.Compilation.BuildManager.CallPreStartInitMethods(String preStartInitListPath, Boolean& isRefAssemblyLoaded) +334
   System.Web.Compilation.BuildManager.ExecutePreAppStart() +178
   System.Web.Hosting.HostingEnvironment.Initialize(ApplicationManager appManager, IApplicationHost appHost, IConfigMapPathFactory configMapPathFactory, HostingEnvironmentParameters hostingParameters, PolicyLevel policyLevel, Exception appDomainCreationException) +746

[HttpException (0x80004005): Could not load file or assembly 'mvcappname' or one of its dependencies. Access is denied.]
   System.Web.HttpRuntime.FirstRequestInit(HttpContext context) +552
   System.Web.HttpRuntime.EnsureFirstRequestInit(HttpContext context) +122
   System.Web.HttpRuntime.ProcessRequestNotificationPrivate(IIS7WorkerRequest wr, HttpContext context) +737

Version Information: Microsoft .NET Framework Version:4.0.30319; ASP.NET Version:4.8.4494.0

已完成的排查操作 #

• 对比正常站点与异常站点的用户权限配置，两者一致
• 重启过服务器及本地开发电脑
• 测试验证：将正常站点的站点名称.dll复制到异常站点后，站点可正常登录
• 确认服务器未做任何配置更改，本地VS2022已更新至最新版本

寻求该问题的排查及解决思路。

内容的提问来源于stack exchange，提问作者Jkulp