运行TOBIAS的BINDetect步骤时遇Matplotlib属性错误求助

使用TOBIAS分析ATAC-seq数据，运行BINDetect模块时执行以下命令：

TOBIAS BINDetect --motifs test_data/motifs.jaspar --signals test_data/Bcell_footprints.bw test_data/Tcell_footprints.bw --genome test_data/genome.fa --peaks test_data/merged_peaks_annotated.bed --peak_header test_data/merged_peaks_annotated_header.txt --outdir BINDetect_test --cond_names Bcell Tcell --cores 4

出现如下报错：

2023-07-05 08:54:45 (488) [INFO]        Writing all_bindetect files
2023-07-05 08:54:45 (488) [INFO]        Creating BINDetect plot(s)
2023-07-05 08:54:45 (488) [INFO]        - Bcell / Tcell (static plot)
Traceback (most recent call last):
  File "/home/usrname/.local/bin/TOBIAS", line 8, in <module>
    sys.exit(main())
  File "/home/usrname/.local/lib/python3.10/site-packages/tobias/TOBIAS.py", line 154, in main
    args.func(args)
  File "/home/usrname/.local/lib/python3.10/site-packages/tobias/tools/bindetect.py", line 819, in run_bindetect
    fig = plot_bindetect(motif_list, clustering, [cond1, cond2], args)
  File "/home/usrname/.local/lib/python3.10/site-packages/tobias/tools/bindetect_functions.py", line 769, in plot_bindetect
    adjust_text(txts, ax=ax1, add_objects=[l], text_from_points=True, arrowprops=dict(arrowstyle='-', color='black', lw=0.5))  #, expand_text=(0.1,1.2), expand_objects=(0.1,0.1))
  File "/home/usrname/.local/lib/python3.10/site-packages/adjustText/__init__.py", line 730, in adjust_text
    ax.annotate(
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/axes/_axes.py", line 699, in annotate
    a = mtext.Annotation(text, xy, xytext=xytext, xycoords=xycoords,
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 1885, in __init__
    Text.__init__(self, x, y, text, **kwargs)
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/_api/deprecation.py", line 454, in wrapper
    return func(*args, **kwargs)
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 183, in __init__
    self.update(kwargs)
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/text.py", line 231, in update
    super().update(kwargs)
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/artist.py", line 1213, in update
    return self._update_props(
  File "/home/usrname/.local/lib/python3.10/site-packages/matplotlib/artist.py", line 1197, in _update_props
    raise AttributeError(
AttributeError: 'Annotation' object has no property 'text_from_points'

已尝试升级matplotlib至3.7.1，卸载重装后问题依旧，使用的是TOBIAS官方测试数据，排除输入文件问题。

错误根源是adjustText库版本不兼容：TOBIAS代码中调用了text_from_points参数，但该参数在新版adjustText中已被移除/更名。可通过以下方法解决：

• 安装兼容版本的adjustText：

pip install adjustText==0.7.3

• 若不需要绘图结果，可直接禁用BINDetect的绘图功能，跳过报错步骤，修改命令如下：

TOBIAS BINDetect --motifs test_data/motifs.jaspar --signals test_data/Bcell_footprints.bw test_data/Tcell_footprints.bw --genome test_data/genome.fa --peaks test_data/merged_peaks_annotated.bed --peak_header test_data/merged_peaks_annotated_header.txt --outdir BINDetect_test --cond_names Bcell Tcell --cores 4 --no-plots

• 升级TOBIAS到最新版本，检查是否已修复该参数兼容问题：

pip install --upgrade tobias

内容的提问来源于stack exchange，提问作者pythonquestions