Snakemake中MultiQC wrapper报错：找不到'imp'模块

背景 #

FastQC规则可正常执行，但MultiQC规则运行失败，报错提示找不到imp模块。此前该规则可正常运行，尝试使用更高版本的wrapper（v2.6.0）后仍出现相同错误。

Snakemake规则代码 #

rule fastqc:
    input:
        "reads/{sample}_trimmed.fq.gz"
    output:
        html="qc/fastqc/{sample}.html",
        zip="qc/fastqc/{sample}_fastqc.zip" # the suffix _fastqc.zip is necessary for multiqc to find the file
    params:
        extra = "--quiet"
    log:
        "logs/fastqc/{sample}.log"
    threads: config["resources"]["fastqc"]["cpu"]
    resources:
        runtime=config["resources"]["fastqc"]["time"]
    wrapper:
        "v1.31.1/bio/fastqc"


rule multiqc:
    input:
        expand("qc/fastqc/{sample}_fastqc.zip", sample=SAMPLES)
    output:
        report("qc/multiqc.html", caption="workflow/report/multiqc.rst", category="MultiQC analysis of fastq files")
    params:
        extra="",  # Optional: extra parameters for multiqc.
        use_input_files_only=True, # Optional, use only a.txt and don't search folder samtools_stats for files
    resources:
        runtime=config["resources"]["fastqc"]["time"]
    log:
        "logs/multiqc/multiqc.log"
    wrapper:
        "v1.31.1/bio/multiqc"

报错信息 #

Traceback (most recent call last):
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/bin/multiqc", line 6, in <module>
    from multiqc.__main__ import run_multiqc
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/__init__.py", line 16, in <module>
    from .multiqc import run
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/multiqc.py", line 30, in <module>
    from .plots import table
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/plots/table.py", line 9, in <module>
    from multiqc.plots import beeswarm, table_object
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/plots/beeswarm.py", line 8, in <module>
    from multiqc.plots import table_object
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/plots/table_object.py", line 9, in <module>
    from multiqc.utils import config, report
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/multiqc/utils/report.py", line 18, in <module>
    import lzstring
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/lzstring/__init__.py", line 11, in <module>
    from future import standard_library
  File "/mnt/4TB_SSD/analyses/CRISPR/test/.snakemake/conda/db6c33339e73e6beea68618300022717_/lib/python3.12/site-packages/future/standard_library/__init__.py", line 65, in <module>
    import imp
ModuleNotFoundError: No module named 'imp'

MultiQC wrapper的Conda环境配置 #

channels:
  - conda-forge
  - bioconda
  - nodefaults
dependencies:
  - multiqc =1.16

疑惑 #

我查询得知imp模块是Python导入机制的一部分，不解为何会出现找不到该模块的情况。

内容的提问来源于stack exchange，提问作者justinian482