如何调整ComplexHeatmap中anno_barplot标题角度以避免重叠？

要将log10(FDR)的注释标题旋转90度以避免重叠，你可以在rowAnnotation中通过annotation_name_rot参数为每个注释单独设置标题旋转角度：给第一个注释指定90度旋转，第二个保持默认水平即可。

修改后的完整代码如下：

aa <- rowAnnotation(
  "log10(FDR)" = anno_barplot(fdr), 
  "log2(Fold Change)" = anno_barplot(fc, gp = gpar(fill = fc_color(fc))),
  width = unit(3, 'cm'),
  gap = unit(10, "points"),
  annotation_name_rot = c(90, 0)  # 第一个注释标题旋转90度，第二个保持水平
)
Heatmap(matrix = mtx, 
        border = T, 
        column_order = ordered_sample, 
        heatmap_legend_param = list(title = "", 
                                    legend_height = unit(4, "cm")), 
        column_names_rot = -90, 
        row_names_gp = gpar(fontsize = 8), 
        column_names_gp = gpar(fontsize = 8), 
        clustering_method_rows = "ward.D2", 
        name = "Scale(exp)", 
        cluster_column_slices = T, 
        row_split = rr_,
        cluster_rows = T, 
        cluster_columns = F, 
        clustering_method_columns = "ward.D2",
        col = htmp_color, 
        right_annotation = aa
)

说明 #

• annotation_name_rot参数接受向量输入，向量长度与注释数量一致，每个元素对应对应注释标题的旋转角度（单位：度）。
• 垂直排列的log10(FDR)标题不会与右侧较长的log2(Fold Change)标题重叠，同时能保持注释区域的布局整洁。

内容的提问来源于stack exchange，提问作者StringAech