使用codonbias的FrequencyOfOptimalCodons类报错，求正确用法及解决方法

问题场景 #

我正在编写用于分析序列密码子使用情况的脚本，使用codon-bias包，调用codonbias.scores.FrequencyOfOptimalCodons类的代码如下：

FOC = cb.scores.FrequencyOfOptimalCodons(ref_seq=sequence_list, genetic_code=11)

其中sequence_list是包含ORF的字符串列表，genetic_code设置为细菌、古菌和植物质体的编码（编号11）。

报错详情 #

运行脚本时触发如下错误：

Traceback (most recent call last):
  File "I:\R&D\Product Research Group\Metabolic&Regulatory modeling\Codon usage\Python scripts\CodonBias CAI analyzer.py", line 117, in <module>
    codon_df, total_orfs, analyzed_orfs = analyze_codon_usage(orfs_file, trna_file)
  File "I:\R&D\Product Research Group\Metabolic&Regulatory modeling\Codon usage\Python scripts\CodonBias CAI analyzer.py", line 71, in analyze_codon_usage
    FOC = cb.scores.FrequencyOfOptimalCodons (ref_seq=sequence_list, genetic_code=11)
  File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\codonbias\scores.py", line 199, in __init__
    self.weights = self.weights.droplevel('aa')
  File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\generic.py", line 943, in droplevel
    new_labels = labels.droplevel(level)
  File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\base.py", line 2155, in droplevel
    levnums = sorted(self._get_level_number(lev) for lev in level)[::-1]
  File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\base.py", line 2155, in <genexpr>
    levnums = sorted(self._get_level_number(lev) for lev in level)[::-1]
  File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\multi.py", line 1660, in _get_level_number
    raise ValueError(
ValueError: The name aa occurs multiple times, use a level number

已确认内容 #

我已经检查过sequence_list中的序列格式和顺序均正常，问题定位到FrequencyOfOptimalCodons类的__init__方法中（对应scores.py文件第199行）。

求助问题 #

请问我的操作哪里出现了错误？

内容的提问来源于stack exchange，提问作者Shlomo Goren